Journal: Biosensors & bioelectronics: X
Article Title: Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine
doi: 10.1016/j.biosx.2025.100658
Figure Lengend Snippet: Principle of SSMG-LAMP assay for high-risk HPV detection. (a) Schematic diagram triple-mode detection in SSMG-LAMP. (b) UV–Vis absorption, (c) fluorescence emission, and (d) cyclic voltammetry plots of HPV + samples and negative controls. (e) Electrophoresis analysis, colorimetric visualization, and fluorescence detection of HPV 16 and 18. (f) Peak value of absorbance (i), fluorescence (ii), and current (iii) of negative and positive samples for colorimetric, fluorescent, and electrochemical detection of HPV 16 and 18, respectively. Negative control with no DNA template and Positive sample with 1200 (HPV 16) or 1000 (HPV 18) copies μL −1 of DNA template. M: DNA ladder, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. The experiment was performed in three replicates.
Article Snippet: HPV 16 (ATCC 45113D), HPV 18 (ATCC 45152D), and HPV 11 (ATCC 45151D) purified plasmid DNA were supplied by American Type Culture Collection (Manassas, VA).
Techniques: Lamp Assay, Fluorescence, Electrophoresis, Negative Control